Speakers - CARWC2025

Abstract

The aging process is a multifaceted phenomenon resulting in declines in bodily functions and increased vulnerability to age-related disorders. [1] In recent years, there has been growing interest in Traditional Chinese Medicine (TCM) principles and the therapeutic potential of natural bioactive molecules, which can modulate the cellular processes implicated in aging [2–5], particularly those from plants and fungi, for their potential in addressing age-related issues and promote healthy aging. Cordyceps sinensis is a notable fungus recognized for its adaptogenic properties, which help the body cope with stress without adverse effects. Previously, it has been demonstrated that it enhances mitochondrial function and energy metabolism, [6] which may contribute to its anti-aging effects. [7]

Although it has been traditionally used to promote longevity, research on its impact on skin aging is minimal. This study investigates an extract of C. sinensis mycelium (CsEx) to assess its effects on longevity markers, including SirT1, ATP, and Pro-Collagen I, in the context of anti-aging interventions. In this study, a Cordyceps sinensis mycelium hydroethanolic extract (CsEx), which was standardized in cordycepin and adenosine using the UHPLC-DAD method, was investigated for its adaptogenic molecular mechanism using in vitro assays on skin cells and a double-blind, placebo-controlled clinical trial involving 40 subjects with various skin aging-related disorders. The CsEx demonstrated activity at a concentration of 0.0006%, significantly increasing sirtuin expression (SirT1: +33%, SirT3: +10%, SirT6: +72%, vs. CTR, p < 0.05) and NAD+ synthesis in HaCat cells (+20% vs. CTR, p < 0.001). Moreover, the CsEx boosted ATP production by 68% in skin cells, correlating with higher skin energy values (+52.0% at D28, p < 0.01) in the clinical trial. Additionally, CsEx notably reduced cytosolic reactive oxygen species (ROS) by 30% in HaCaT cells (p < 0.05) and enhanced collagen production both in vitro (+69% vs. CTR, p < 0.01) and in vivo (+10% vs. D0, p < 0.01), confirmed by ultrasound examination. Furthermore, CsEx’s stimulation of fibroblasts and its antioxidant and energizing properties led to a significant reduction in wrinkles by 28.0% (D28, p < 0.001).

This study underscores Cordyceps sinensis hydroethanolic extract’s potential in regulating skin cell energy metabolism and positively influencing the mechanisms associated with skin longevity control, merging TCM with modern science.